EPO Quantification Kit

The EPO Quantification Kit from MAIIA Diagnostics is a lateral flow immunoassay intended for fast and easy quantification of EPO in buffer solutions or urine specimens.

An immobilized anti-EPO antibody is used to bind and concentrate EPO in a thin capture zone on the lateral flow strip. Anti-EPO Carbon Black Nano-Strings are then applied for detection and quantification of the bound analyte, yielding a black to grey signal. The intensity of blackness in the capture zone is proportional to the amount of bound EPO, and hence the concentration of EPO in the sample can be estimated using an EPO standard curve.

The procedure is rapid and sensitive, and requires no additional equipment when used for semi-quantitative detection.
The test can be made quantitative by placing the strips in a flatbed scanner and quantifying the signal intensity by our novel software, MAIIAcalc.

The short reaction time (15 min.) makes it suitable to use for measuring EPO in ultra filtered urine retentate in order to obtain the optimal EPO amount for application on the IEF gel used for analysis of EPO isoform patterns.